Comparison of positive and negative ion detection of tea catechins using tandem mass spectrometry and ultra high performance liquid chromatography

摘要: Abstract Tea catechins are an important group of natural compounds associated with health promoting effects and desired commodities for the growing market dietary supplements functional foods. Consequently these attract more interest research groups worldwide. A reliable quantitative analysis tea is essential human intervention studies, manufacturers quality control by authorities. UHPLC–ESI-MS/MS analytical method was chosen due to rapid runtime, high sensitivity selectivity. The chromatographic separation eight achieved within 2.5 min on C 18 BEH column (100 mm × 2.1 mm i.d.; 1.7 μm), whilst gradient elution mode employed using water:methanol mobile phase addition volatile organic acid. concentration acids in optimised range 0.01–0.1% (v/v). High sensitivities were positive (10.2-16.8 fmol/inj.) negative ion detection (102.1-168.1 fmol/inj.), through accurate complex tuning MS parameters. UHPLC-ESI-MS/MS validated terms linearity (>0.9997; >0.9990), (0.02–2.40 mg L −1 ; 0.15-24.00 mg L ), LOD (3.0–4.8 μg L 30.1–48.0 μg L LOQ (9.9–15.8 μg L 150.5-240.0 μg L intra-day precision (4.4-7.1% RSD; 3.3-5.1% RSD), accuracy (94.06-113.7%; 89.5-108.4%), retention time repeatability (0.0-0.5% 0.0-0.6% peak area (1.2-4.0% 2.4-3.5% RSD) modes, respectively. statistical comparison results obtained performed.