Analysis of MHC class II and monogenic eye diseases in the Scandinavian wolf population

摘要: ABSTRACT. In the late 1960’s all wolves in Scandinavia went extinct wild due to extensive hunting. In 1983 two new immigrated and founded, together with a third immigrant 1991, population that exists today. Due fact originates from less than handful of individuals, genetic variation is small. Between years 1973 2011, 23 have been sampled for blood tissue were used this study. A hereditary form blindness without known etiology has reported among captive Sweden. an attempt find disease-causing gene, study analyzed presence muta-tions following monogenic eye diseases; Gr_PRA1, Type-A PRA, Cord1, Prcd-PRA PLL. The analysis diseases was performed either real time PCR (TaqMan) or capillary electrophoresis (ABIPrism 3100). To gain further understanding about ge-netic complexity degree Scandinavian wolf population, also sequenced their MHC class II loci (DLA-DRB1, DLA,DQA1 DLA-DQB1). tests tested normal, but does not exclude possibility individuals carry another mutation causing blindness. II, eight haplotypes found including DRB1, six DQA1 seven DQB1 alleles. Compared common dog breed half variation. Because lack variation, license hunting should be abandoned favor careful testing depending on result, cautious selection what animals eu-thanize. SAMMANFATTNING. I slutet av sextiotalet utrotades alla vargar i Skandinavien pa grund omfattande jakt. immigrerade tva till Sverige och grundlade tillsammans med ytterligare en ar 1991 den vargpopulation som finns idag. Eftersom populationen etablerades sa fa genetiska variationen liten. Mellan aren 2011 provtogs var-gar blod vavnad varav anvandes denna studie . Vargar fangenskap lider arftlig blindhet okand genetisk harkomst. I ett forsok att finna orsakande mutationen analyserades provtagna sjukdomsorsakande mutationer foljande monogena ogonsjukdomar; Typ-A Analysen ogonsjukdomarna genomfordes antingen realtids-PCR eller kapillarelektrofores. For kunskap om hos vargarna utfordes sekvensering klass tester ogonsjukdomar var normala. Hos fanns atta haplotyper sex sju alleler. Jamfort vanlig hundras har Skandinaviska vargpopulationen ungefar halften variationen. Pa begransade borde licensjakt overges forman grundliga Beroende resultat skall djur noggrant utvaljas avlivning, eftersom det foreligger betydande risker kan resultera minskad