Realistic simulations reveal extensive sample-specificity of RNA-seq biases

摘要: In line with the importance of RNA-seq, bioinformatics community has produced numerous data analysis tools incorporating methods to correct sample-specific biases. However, few advanced simulation exist enable benchmarking competing correction methods. We introduce first framework reproduce properties individual RNA-seq runs and, by applying it on several datasets, we demonstrate accounting for sample-specificity in realistic simulations.