Tubular sclerosis rather than the smear layer impedes dye penetration into the dentine of endodontically instrumented root canals.

摘要: AIM: To evaluate the effect of different root canal irrigating regimes on dentine penetration Patent Blue dye. METHODOLOGY: Eighty extracted single-rooted human mandibular premolar teeth with narrow canals were prepared using ProFile instruments. After each instrument, irrigated 1% sodium hypochlorite. Subsequently, randomly assigned to receive a 10 mL rinse aqueous 17% (w/v) ethylenediaminetetraacetic acid or tap water for 2 min, followed by final 2% dye solution min (eight groups, n = per group). Teeth then horizontally sectioned 3, 6 and 9 mm from apex. Sections digitally photographed was calculated as percentage total area NIH Image J. Values compared one-way anova Bonferroni correction alpha-type error set at <0.05. Representative tooth sections all groups further analysed scanning electron microscopy. RESULTS: No significant impact protocols found. Dye significantly (P < 0.001) greater in coronal than middle, middle apical thirds. When observed microscopically, irrigant independent presence smear layer, but rather function tubular sclerosis. CONCLUSIONS: Tubular sclerosis, physiological phenomenon that starts third decade life region advances coronally age, main factor influencing penetrability dentine.