Solubilization of the platelet plasma membrane serotonin transporter in an active form.

摘要: The activity of the serotonin transporter platelet plasma membrane may be assessed by its ability to bind [N-methyl-3H]imipramine, even in presence detergents which disrupt and inactivate transport. Of 21 were tested for their solubilize an active form, some, such as Triton X-100, imipramine-binding a manner is difficult or impossible reverse, while inactivation others, cholate, readily reversed gel filtration. do not imipramine binding, most, Tween series, also fail significant amounts transporter. all tested, only one, digitonin, solubilized specific activity. Imipramine binds digitonin-solubilized with slightly reduced affinity, but other respects binding intact membranes identical. Thus, is, both cases, saturable Na+-dependent, serotonin. Furthermore, inhibitors transport serotonin-mediated aggregation block soluble preparation. Sodium-dependent extract insensitive K+, suggesting that separate sites on mediate effects Na+ K+.