Ultrastructural quantification of cell death after injurious compression of bovine calf articular cartilage

摘要: Abstract Objective: It has been suggested that chondrocyte death by apoptosis may play a role in the pathogenesis of cartilage destruction osteoarthritis, but results in-vivo and in-vitro investigations have conflicting. To investigate further cell our model for traumatic joint injury, we performed quantitative analysis electron microscopy (EM) morphology after injurious compression. For comparison, TUNEL assay was also performed. Design: Articular explant disks were harvested from newborn calf femoropatellar groove. The subjected to compression (50% strain at rate 100%/s), incubated 3 days, then fixed morphological analysis. Results: By TUNEL, increased 7±2% unloaded controls 33±6% injury ( P =0.01; N =8 animals). EM, 5±1% 62±10% injured =0.02, =5 Analysis EM identified dead cells disks, 97% apoptotic morphology. Conclusions: These confirm significant increase suggest most observed here an process.