Tandem separation of labelled human blood platelet membrane glycoproteins by anion-exchange and gel fast protein liquid chromatography.
作者： J.L. McGregor , P. Clezardin , M. Manach , M. Dechavanne
DOI: 10.1016/S0021-9673(01)87444-6
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摘要: Studies were made of the separation surface-labelled platelet glycoproteins, solubilized in a non-ionic detergent (Berol 185), on Mono Q anion-exchange column, coupled to Superose 12 gel fast protein liquid chromatographic column. Peaks eluted from and columns subjected electrophoresis sodium dodecyl sulphate-polyacrylamide gels presence (non-reducing conditions) or absence (reducing disulphide bridges. Labelled electrophoresed glycoprotein bands rendered visible by fluorography indirect autoradiography. Platelet membrane glycoproteins Ib, IIb, IIIa, IIIb, V IX identified their apparent molecular weights surface labelling characteristics. It was concluded that tandem chromatography can be used separate proteins rapidly with good resolution.
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