Inefficient nodulation of chickpea ( Cicer arietinum L.) in the arid and Saharan climates in Tunisia by Sinorhizobium meliloti biovar medicaginis

作者: Samir Ben Romdhane , Mohamed Elarbi Aouani , Ridha Mhamdi

DOI: 10.1007/BF03175044

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摘要: The aim of this work is to study the genetic diversity and symbiotic effectiveness natural populations rhizobia nodulating chickpea (Cicer arietinum L.) in six locations South Tunisia, where had never been cultivated. Nodules were observed only two soil samples from Gafsa (0.8 nodules per plant) Tataouine (2 plant). PCR-RFLP typing 165 rRNA genes 42 isolates indicated that all analysed strains showed same ribotype as reference strainSinorhizobium meliloti RCR2011. These induced ineffective on andMedicago sativa; however onMedicago laciniata effective. Analysis by PCR-RFLP, thenifDK spacer suggested belong biovarmedicaginis ofS. meliloti. present paper is, our knowledge, first report showing selectively nodulated under conditions a specific biovar specificity toM. laciniata. interaction well impact inefficient nodulation cultivation needs be investigated further.

参考文章(28)
N. Amarger, V. Macheret, G. Laguerre, Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris Nodules International Journal of Systematic and Evolutionary Microbiology. ,vol. 47, pp. 996- 1006 ,(1997) , 10.1099/00207713-47-4-996
Y. D. GAUR, A. N. SEN, CROSS INOCULATION GROUP SPECIFICITY IN CICER‐RHIZOBIUM SYMBIOSIS New Phytologist. ,vol. 83, pp. 745- 754 ,(1979) , 10.1111/J.1469-8137.1979.TB02305.X
S. M. NOUR, J.-C. CLEYET-MAREL, P. NORMAND, M. P. FERNANDEZ, Genomic heterogeneity of strains nodulating chickpeas (Cicer arietinum L.) and description of Rhizobium mediterraneum sp. nov. International Journal of Systematic and Evolutionary Microbiology. ,vol. 45, pp. 640- 648 ,(1995) , 10.1099/00207713-45-4-640
S. M. NOUR, M. P. FERNANDEZ, P. NORMAND, J.-C. CLEYET-MAREL, Rhizobium ciceri sp. nov., Consisting of Strains That Nodulate Chickpeas (Cicer arietinum L.) International Journal of Systematic and Evolutionary Microbiology. ,vol. 44, pp. 511- 522 ,(1994) , 10.1099/00207713-44-3-511
E. S. P. Bromfield, L. R. Barran, Promiscuous nodulation of Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala by indigenous Rhizobium meliloti Canadian Journal of Microbiology. ,vol. 36, pp. 369- 372 ,(1990) , 10.1139/M90-065
Bouchaib Khbaya, Marc Neyra, Philippe Normand, Karim Zerhari, Abdelkarim Filali-Maltouf, Genetic Diversity and Phylogeny of Rhizobia That Nodulate Acacia spp. in Morocco Assessed by Analysis of rRNA Genes Applied and Environmental Microbiology. ,vol. 64, pp. 4912- 4917 ,(1998) , 10.1128/AEM.64.12.4912-4917.1998
W G Weisburg, S M Barns, D A Pelletier, D J Lane, 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. ,vol. 173, pp. 697- 703 ,(1991) , 10.1128/JB.173.2.697-703.1991