Novel methodology to evaluate the effect of residual moisture on epoxy resin sealer/dentine interface: a pilot study.

摘要: Gibby SG, Wong Y, Kulild JC, Williams KB, Yao X, Walker MP. Novel methodology to evaluate the effect of residual moisture on epoxy resin sealer/dentine interface: a pilot study. International Endodontic Journal 44, 236–244, 2011. Abstract Aim  To interface associated with an sealer using combination Goldner’s trichrome stain (GTS) and scanning electron microscopy (SEM) verify use experimental methodology. Methodology  Extracted human maxillary incisors (6) were subjected root canal treatment. Subsequent pulp removal, instrumentation smear layer removal EDTA NaOCl, teeth randomly equally assigned ‘wet’ or ‘dry’ group. The group was desiccated (95% ethanol/suction/paper points/air-drying), whilst treated saline rinse/suction/single paper point. Canals then filled epoxy-based warm vertical gutta-percha compaction. After 7-day storage at 37 °C, roots from each sectioned into apical, middle coronal horizontal subsections that cut split paired halves evaluated GTS SEM. With sections, hybrid tubular penetration measured (n = 15 measurements/intracanal location/condition) two-factor repeated measures analysis variance. SEM qualitative sections included as complementary confirmation analyses. Results  In dry wet groups, there no conspicuous layer, irrespective location. However, specimens exhibited more uniform distribution deeper in third (P < 0.05). contrast, decreased tubule apical third, regardless condition (P < 0.05). Conclusions  (combination SEM) can be used intracanal interface. data indicated thorough drying system may result improved penetration, especially thirds canals.