Essai de mise en évidence d'un rôle régulateur des tRNA dans la synthèse des protéines de l'œuf de poule: I. Étude comparative des acides ribonucléiques de transfert extraits du foie et de l'oviducte de poule en ponte
作者： P. Gerlinger , M.A. Le Meur , D. Illinger , J.P. Ebel
DOI: 10.1016/S0300-9084(74)80069-6
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摘要: Summary The liver and the oviduct of laying hen are target organs oestrogens synthesize egg yolk white respectively. aminoacyl-tRNA's from these have been systematically compared. Control experiments conducted to show that a tRNA extracted 105 000 × g supernatant is not degraded nor contaminated with other A 260 adsorbing material it represents whole cytoplasmic tRNA. aminoacylation levels for several aminoacids, which different between two organs, were verified by analyzing reaction parameters, particular emphasis on enzyme amino acid concentration in medium. fractionation profiles compared reversed phase chromatography (RPC 5). More than half quantitative differences relative proportion their isoaccepting species.
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