Low-intensity vibrations normalize adipogenesis-induced morphological and molecular changes of adult mesenchymal stem cells.

摘要: Bone marrow mesenchymal stem cells that are committed to adipogenesis were exposed daily high-frequency low-intensity mechanical vibrations understand molecular, morphological and ultrastructural adaptations signals during adipogenesis. D1-ORL-UVA mouse bone cultured with either growth or adipogenic medium for 1 week. Low-intensity vibration (15 min/day, 90 Hz, 0.1 g) applied one group of cells, while the other served as a sham control. Cellular viability, lipid accumulation, ultrastructure morphology determined MTT, Oil-Red-O staining, phalloidin staining atomic force microscopy. Semiquantitative reverse transcription polymerase chain reaction showed expression profile genes responsible cells. increased viability in culture was reduced significantly compared quiescent controls. also normalized effects condition on cell morphology, including area, perimeter, circularization actin cytoskeleton. Furthermore, some markers significantly. Mesenchymal sensitive responsive loads, but debilitating conditions such aging obesity may steer toward Here, application partially neutralized induction suggesting these provide an alternative and/or complementary option reduce fat deposition.