Sex Genotyping of Archival Fixed and Immunolabeled Guinea Pig Cochleas.
作者： Frédéric F. Depreux , Lyubov Czech , Donna S. Whitlon
DOI: 10.1038/S41598-018-23491-3
关键词:
摘要: For decades, outbred guinea pigs (GP) have been used as research models. Various past studies using measures that, unknown at the time, may be sex-dependent, but from which today, archival tissues all that remain. We aimed to provide a protocol for sex-typing pig tissue, whereby experiments could re-evaluated sex effects. No PCR sex-genotyping protocols existed GP. found published sequence of GP Sry gene differed in two separate stocks. sequences other species deduce primers Sry. After developing genomic DNA extraction archival, fixed, decalcified, immunolabeled, cochlear half-turns, we multiplex assay (Y-specific Sry; X-specific Dystrophin) assign tissue old 3 years. This procedure should allow reevaluation prior various areas effects on experimental outcomes.
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