Targeted killing of cancer cells in vivo and in vitro with EGF-directed carbon nanotube-based drug delivery.
作者： Ashwin A. Bhirde , Vyomesh Patel , Julie Gavard , Guofeng Zhang , Alioscka A. Sousa
DOI: 10.1021/NN800551S
关键词:
摘要: Carbon nanotube-based drug delivery holds great promise for cancer therapy. Herein we report the first targeted, in vivo killing of cells using a drug-single wall carbon nanotube (SWNT) bioconjugate, and demonstrate efficacy superior to nontargeted bioconjugates. First line anticancer agent cisplatin epidermal growth factor (EGF) were attached SWNTs specifically target squamous cancer, control was SWNT-cisplatin without EGF. Initial vitro imaging studies with head neck carcinoma (HNSCC) overexpressing EGF receptors (EGFR) Qdot luminescence confocal microscopy showed that SWNT-Qdot-EGF bioconjugates internalized rapidly into cells. Limited uptake occurred EGF, blocked by siRNA knockdown EGFR cells, revealing importance EGF-EGFR binding. Three color, two-photon intravital video injected live mice selectively taken up b...
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