Effectiveness of nine polymorphic microsatellite markers in parentage testing in Posavina, Croatian Coldblood and Lipizzaner horse breeds in Croatia

摘要: Abstract The global biodiversity crisis extends to autochthonous local breeds of livestock. There is an increasing danger that these rare become extinct and with them their locally adapted gene pool. Modern molecular tools such as parentage testing using microsatellite genotyping are powerful in guiding management conservation. We tested nine markers three Croatian horse obtained high exclusion probabilities (EPs) for the most common test scenario ‘one parent offspring known other tested’ (99.9% Posavina Coldblood 99.3% Lipizzaner), despite Lipizzaner has overall lower genetic variability at loci. To a useful tool breed countries developing economies, screening systems must be designed statistically yet economically viable. Therefore, suite six can run two multiplex which still gives (99.5% 98% Lipizzaner) was chosen.