Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation

摘要: The advancement of DNA-based bionanotechnology requires efficient strategies to functionalize DNA nanostructures in a specific manner with other biomolecules, most importantly peptides and proteins. Common DNA-functionalization methods rely on laborious covalent conjugation between proteins or peptides. Pyrrole-imidazole (Py–Im) polyamides, based natural minor groove DNA-binding small molecules, can bind sequence fashion. In this study, we explore the use Py–Im polyamides for addressing non-covalent manner. A generic synthetic approach native chemical ligation was established that allows both recombinant polyamides. effect polyamide binding investigated by Surface Plasmon Resonance (SPR). Although synthesis different protein-Py–Im-polyamide conjugates successful, attenuation affinity observed, particular conjugates. practical structures an orthogonal but manner, therefore, remains be established.