Development of Estrogen-Responsive Transgenic Medaka for Environmental Monitoring of Endocrine Disrupters
作者： Zhiqiang Zeng , Tao Shan , Yan Tong , Siew Hong Lam , Zhiyuan Gong
DOI: 10.1021/ES050728L
关键词:
摘要: To develop a transgenic fish system to monitor environmental pollution, we generated mvtg1:gfp medaka line, in which the gfp reporter gene was under control of vitellogenin1 (mvtg1) promoter. In this GFP exclusively expressed liver mature adult female. Male and juvenile did not express but could be induced after exposure 17-β-estradiol (E2). Concurrent accumulation mvtg1 mRNAs observed during both development estrogen treatment, indicating that transgene faithfully Dose- time-dependent induction expression by E2 investigated male fish. The lowest-observed-effect concentration (LOEC) induce 0.5 μg/L observation live 0.05−0.1 dissection-exposed 30 day experiment. within 36 h treatme...
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