Adhesion, invasion and intracellular replication of Salmonella typhimurium in a murine hepatocyte cell line. Effect of cytokines and LPS on antibacterial activity of hepatocytes.

摘要: Elimination of pathogenic microorganisms in the liver may be an important effector mechanism host defenses. In this paper we describe adhesion, invasion and multiplication Salmonella typhimurium a murine embryonic hepatocyte cell line (ATCC TIB-73). Monolayers hepatocytes treated with recombinant IFN gamma, IL1 beta, LPS exhibit antibacterial activity against intracellular Salmonella. The dynamic infection process stimulated vs unstimulated was determined by counting number survival bacteria monolayers at 4 28h after gentamicin added to infected cells. is able adhere, invade replicate inside hepatocytes. maximum cell-associated approximately 15 per cell, whereas invasive capacity 0.003 hepatocyte. Stimulated cultures display compared controls. does not seem mediated nitric oxide (NO) since inhibition NO production using NG-Monomethyl-L-Arginine did revert activity. Also, high amounts induced adding L-Arginine enhance