Vinblastine suppresses dynamics of individual microtubules in living interphase cells.

摘要: We have characterized the effects of vinblastine on dynamic instability behavior individual microtubules in living BS-C-1 cells microinjected with rhodamine-labeled tubulin and found that at low concentrations (3-64 nM), potently suppresses without causing net microtubule depolymerization. Vinblastine suppressed rates growth shortening, decreased frequency transitions from or pause to also called catastrophe. In vinblastine-treated cells, both average duration a (a state attenuated dynamics where neither nor shortening could be detected) percentage total time spent were significantly increased. dynamicity, measure overall activity microtubules, reducing this parameter by 75% 32 nM. The present work, consistent earlier vitro studies, demonstrates kinetically caps ends supports hypothesis potent chemotherapeutic action as an antitumor drug is suppression mitotic spindle dynamics. Further, results indicate molecules bind can regulate suggest endogenous regulators work similar mechanisms may exist cells.