Post-ischaemic restituted intestinal mucosa is more resistant to further ischaemia than normal mucosa in the pig

摘要: Objective. Ischaemic preconditioning may protect the intestine from subsequent prolonged ischaemia. This study evaluates whether a much longer initial ischaemia, encountered clinically, modify intestinal resistance to further ischaemia in pig model. Material and methods. After cross‐clamping of superior mesenteric artery for 1 h, was either reperfused 8 h or second 1 h performed at 4 h reperfusion. Based on microarray analysis samples 1, 4 reperfusion, mRNA selected genes measured with QRT‐PCR. Results. The first ischaemic period caused exfoliation surface epithelial cells basement membrane comprising about 90 % villi tips, marked increase permeability depletion ATP. challenge 30 % less denudation (p = 0.008), no 0.008) ATP 0.039). mRNAs superoxide dismutase 2, heat s...