Keratin nanoparticles co-delivering Docetaxel and Chlorin e6 promote synergic interaction between chemo- and photo-dynamic therapies

摘要: Abstract The combination of chemotherapy and photodynamic therapy (PDT) is considered a valuable strategy for increasing therapeutic response in cancer treatment, the re-formulation pharmaceuticals biocompatible nanoparticles (NPs) particularly appealing possibility co-loading drugs exerting cytotoxicity by different mechanisms, with aim to produce synergic effects. We report in-water synthesis novel keratin-based nanoformulation co-delivery antimitotic Docetaxel (DTX) photosensitizer Chlorin e6 (Ce6). drug-induced aggregation method allowed formation monodisperse NPs (DTX/Ce6-KNPs) an average diameter 133 nm loaded drug ratio 1:1.8 Ce6 vs DTX. efficacy DTX/Ce6-KNPs was investigated vitro monolayers spheroids DTX-sensitive HeLa (HeLa-P) DTX-resistant (HeLa-R) cells. In monolayers, cytotoxic effects toward HeLa-P cells were comparable those induced free DTX + Ce6, while HeLa-R KNPs produced interaction between PDT. Moreover, as respect monotherapies, stronger both multicellular reduced their volumes up 50%. Overall, results suggest that are very promising systems chemotherapeutics PSs, favoring interactions PDT chemotherapy.