Expression of α-Amylase in Cultured Callus of French Bean

摘要: α-Amylase (EC 3.2.1.1) expression was found in calli of French bean (Phaseolus vulgaris L. cv Goldstar). We examined enzyme activity the to investigate influence gibberellin and sugars on expression. After subculture calli, α-amylase decreased, then increased at a stationary phase callus growth. Exogenous application an inhibitor synthesis, uniconazole, did not have any significant effects Sugar starvation activity, while addition metabolizable sugars, such as sucrose, glucose maltose, medium repressed Addition 6% mannitol, non-metabolizable sugar, induced higher compared 3% mannitol. This result suggests that osmotic stress enhances calli. Furthermore, high concentrations agar It is probable prevented incorporation nutrient into