Hyperosmotic stress rapidly generates lyso-phosphatidic acid in Chlamydomonas.

摘要: Plant cells are continuously exposed to environmental stresses such as hyper-osmolarity, and have respond in order survive. When 32P-labelled Chlamydomonas moewusii were challenged with NaCl, the formation of a new radiolabelled phospholipid was stimulated, which barely detectable before stimulation. The identified lyso-phosphatidic acid (LPA), only lyso-phospholipid be accumulated. increase LPA dose- time-dependent. other osmotically active compounds used, also induced similar kinetics, although salts better inducers than non-salts. At least part generated by phospholipase A2 (PLA2) hydrolysing phosphatidic (PA). This claim is based on PA preceding production, PLA2 inhibitors decreasing accumulation promoting conversion diacylglycerol pyrophosphate. latter another metabolic derivative that implicated cell signalling. involvement multiple lipid-signalling pathways hyperosmotic stress responses discussed.