Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo.

摘要: Potential DNA replication accessory factors from the yeast Saccharomyces cerevisiae have previously been identified by their ability to bind polymerase alpha protein affinity matrices (J. Miles and T. Formosa, Proc. Natl. Acad. Sci. USA 89:1276-1280, 1992). We now used genetic methods characterize gene encoding one of these alpha-binding proteins (POB1) determine whether it plays a role in vivo. find that cells lacking POB1 are viable but display constellation phenotypes indicating defective metabolism. Populations accumulate abnormally high numbers enlarged large-budded with single nucleus at neck bud. The average content population is shifted toward G2 value. These two indicate while bulk completed without POB1, mitosis delayed. Deleting also causes elevated levels both chromosome loss recombination, enhances temperature sensitivity mutant genes, increased UV radiation functional RAD9 checkpoint gene, an probability death carrying mutation MEC1 gene. sequence indicates identical CTF4 (CHL15) screens for mutations diminish fidelity transmission. consistent metabolism strongly suggest this accurately duplicating genome