Biocatalytic Hydrolysis of 3‐Hydroxyalkanenitriles to 3‐Hydroxyalkanoic Acids

摘要: Microbial catalysts having a combination of nitrile hydratase and amidase activities had significantly-higher specific activity for hydrolysis 3-hydroxyalkanenitriles than microbial nitrilase catalysts. Comamonas testosteroni 22–1, Dietzia sp. ADL1 5-MGAM-4D hydratase/amidase biocatalysts each hydrolyzed 3-hydroxyvaleronitrile to 3-hydroxyvaleric acid (as the ammonium salt) in 99–100% yields, but consecutive batch reactions with catalyst recycle, alginate-immobilized C. superior enzyme stability volumetric productivity. In series 85 biocatalyst recycle production 1.0 M acid, recovered final reaction were 29% 40%, respectively, initial activities. The productivity this was 670 g acid/g dry cell weight (50 g bead), 44 g 3-HVA/L/h. Similar results obtained 3-hydroxybutryonitrile 3-hydroxypropionitrile corresponding 3-hydroxyalkanoic salts.