An Experimental Study on Apoptosis of cultivated Wild Ginseng Distilled Herbal Acupuncture by Concentration Level

摘要: Objective : In order to measure the efficacy of cultivated wild ginseng distilled herbal acupuncture by concentration level, we`ve treated A549 human lung cancer lines with different concentrations and examined mRNA proteins which take parts in apoptosis. Methods were various levels cell toxicity was carefully examined. From analysis DNA fragmentation, RT-PCR Western blot, manifestation are associated apoptosis inspected. Results The following results obtained on after administering acupuncture. 1. Measuring cells, strong detected at high level(1000ul, 1200ul), but no consistent dependent reliance detected. 2. Through we able confirm destruction all groups. 3. Experiment groups showed inhibition Bcl-2 COX-2 Protein whileas increase Bax shown. 4. Manifestation p21, p53, Cyclin E, Dl confirmed 5. Extrication Cytochrome C groups, as well increased activity enzyme caspase-3 caspase-9, PARP fragmentation confirmed. Conclusion According results, can deduce induced Apoptosis. At fixed decrease COX-2, Bax. Since increases Dl, it affects cellular cycle through these phenomena, consider extrication C, caspase, results.