A simple method for activating the platelets used in microfluidic platelet aggregation tests: Stirring-induced platelet activation.

摘要: High-shear stimulation is well known as one of the key factors affecting platelet activation and aggregation, which can lead to formation a thrombus. In our previous studies, we introduced migration distance-based function analysis in microfluidic system. this study, set out examine effects stirring on shear-induced aggregation chamber system by using rotating stirrer. We found that stirrer caused not only rotational shear flow but also strong radial secondary flow. The latter led efficient mixing chamber. Moreover, generation stress, magnitude be controlled activate platelets. Activated platelets tend aggregate themselves. maximum was observed at critical rate 3100 s−1, regardless shape. Furthermore, time taken attain significantly shortened when wide (30 s) instead narrow (180 s). When flat stirrer, non-uniform field resolved with flow-induced mixing; thus, most were homogenously activated. stirring-induced mechanism experimentally confirmed for test while monitoring distance until channel occluded. Our findings indicate present system, consisting confined chamber, provides effective activating inducing aggregates.