Human T Cell Priming Assay: Depletion of Peripheral Blood Lymphocytes in CD25 + Cells Improves the In Vitro Detection of Weak Allergen-Specific T Cells
作者： Marc Vocanson , Amine Achachi , Virginie Mutez , Magalie Cluzel-Tailhardat , Béatrice Le Varlet
DOI: 10.1007/978-3-0348-0726-5_7
关键词:
摘要: To develop an in vitro assay that recapitulates the key event of allergic contact dermatitis (ACD), is priming effector T cells by hapten-presenting dendritic cells, and then allows for sensitive detection chemical allergens represents a major challenge. Classical human cell assays (hTCPA) have been developed past, using hapten-loaded monocyte-derived (MDDCs) as antigen-presenting peripheral blood lymphocytes (PBLs) responding were not efficient to prime common with moderate/weak sensitizing properties. Recent progress understanding regulatory mechanisms ACD shown requires uptake immunogenic DCs it controlled several subsets including CD25+ Tregs. We therefore analyzed various parameters involved allergen-specific activation showed hampered immune comprising CD1aneg DCs, CD56+ cells.
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