The effect of cationic charge on release of eosinophil mediators

摘要: Background In patients with atopic diseases, cationic-charged eosinophil proteins are deposited in inflamed tissues. Although the role of cytokines cell activation is well established, presence tissue can also be an important factor inflammatory function. Objectives We sought to determine whether increased cationic charge seen microenvironment plays a eosinophils. Methods Human eosinophils were incubated Sepharose beads coated or anionic compounds and absence cytokine cocktail (IL-3, IL-5, GM-CSF) simulate milieu inflammation. Eosinophil peroxidase eosinophil-derived neurotoxin (EDN) release compared morphology expression CD18, as determined by means confocal microscopy. Results Cytokines positively charged caused greater (lysine coated, 44.2 nmol/L; compound 48/80, 40.0 EDN 49.1 nmol/L) than alone (14.9 nmol/L). Beads heparin, dextran sulfate, aspartic acid did not show this effect. was induced lysine-coated (67.1 ng/100 μL) blocked heparin. incubation wortmannin, genistein, src kinase inhibitor PP1 signaling. Eosinophils adherent but anionic-charged polarization CD18 toward bead's surface. Conclusion Cationic-charged surfaces induce mediator increasing density available at target