A comparison between cytokine- and bead-stimulated polyclonal T cells: the superiority of each and their possible complementary role

摘要: Cytokine-induced killer (CIK) cells and T expanded by co-stimulation with beads presenting anti-CD3 -CD28 antibodies are both polyclonal under intensive laboratory clinical studies, but there has not been any direct comparison between both. We compared the expansion, memory cell subsets cytotoxicity for in parallel two methods. Bead-stimulated showed superior expansion as to CIK on D14 of culture. consisted a significantly higher CD4+ subset lower CD8+ cells, well proportion less terminally differentiated homing molecules. On other hand, exhibited against myelomonocytic leukemia lines (THP-1 U937) RCC (786.0 CaKi-2). The THP-1 was 58.1 % 8.3 % bead-stimulated at E:T 10:1 (p < 0.01). Cytotoxicity correlated positively CD8 culture independent NKG2D recognition susceptible targets. Polyclonal different methods exhibit characteristics which may define specific role each scenario. postulate that more potent offer short term benefit while sustained immune response.