Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker.
作者： K Kim , D Soldati , J. Boothroyd
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摘要: A system for stable transformation of Toxoplasma gondii tachyzoites was developed that exploited the susceptibility to chloramphenicol. Introduction chloramphenicol acetyltransferase (CAT) gene fused flanking sequences followed by selection resulted in parasites stably expressing CAT. construct incorporating tandemly repeated gene, B1, targeted efficiently its homologous chromosomal locus. Knockout single-copy ROP1, also successful. Stable should permit identification and analysis genes important interaction this opportunistic parasite with host.
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