In-cell recordings by extracellular microelectrodes
作者： Aviad Hai , Joseph Shappir , Micha E Spira
DOI: 10.1038/NMETH.1420
关键词:
摘要: Current extracellular multisite recordings suffer from low signal-to-noise ratio, limiting the monitoring to action potentials, and preclude detection of subthreshold synaptic potentials. Here we report an approach induce Aplysia californica neurons actively engulf protruding microelectrodes, providing 'in-cell recordings' potentials with ratio that matches conventional intracellular recordings. Implementation this may open new vistas in neuroscience biomedical applications.
nature.com
sci-hub.se 参考文章(17)
Craig H. Bailey, Eric R. Kandel, Synaptic Remodeling, Synaptic Growth and the Storage of Long-Term Memory in Aplysia Progress in Brain Research. ,vol. 169, pp. 179- 198 ,(2008) , 10.1016/S0079-6123(07)00010-6
P. FROMHERZ, Three Levels of Neuroelectronic Interfacing: Silicon Chips with Ion Channels, Nerve Cells, and Brain Tissue Annals of the New York Academy of Sciences. ,vol. 1093, pp. 143- 160 ,(2006) , 10.1196/ANNALS.1382.011
M.E. Spira, A. Dormann, U. Ashery, M. Gabso, D. Gitler, D. Benbassat, R. Oren, N.E. Ziv, Use of Aplysia neurons for the study of cellular alterations and the resealing of transected axons in vitro. Journal of Neuroscience Methods. ,vol. 69, pp. 91- 102 ,(1996) , 10.1016/S0165-0270(96)00024-6
Ariel Cohen, Joseph Shappir, Shlomo Yitzchaik, Micha E. Spira, Reversible transition of extracellular field potential recordings to intracellular recordings of action potentials generated by neurons grown on transistors. Biosensors and Bioelectronics. ,vol. 23, pp. 811- 819 ,(2008) , 10.1016/J.BIOS.2007.08.027
Martin Jenkner, Peter Fromherz, Bistability of Membrane Conductance in Cell Adhesion Observed in a Neuron Transistor Physical Review Letters. ,vol. 79, pp. 4705- 4708 ,(1997) , 10.1103/PHYSREVLETT.79.4705
Bertil Hille, Ionic channels of excitable membranes ,(1984)
Norio Akaike, Nobutoshi Harata, None, Nystatin Perforated Patch Recording and Its Applications to Analyses of Intracellular Mechanisms Japanese Journal of Physiology. ,vol. 44, pp. 433- 473 ,(1994) , 10.2170/JJPHYSIOL.44.433
Alessia Mortari, Abbas Maaroof, Donald Martin, Michael B Cortie, None, Mesoporous gold electrodes for sensors based on electrochemical double layer capacitance Sensors and Actuators B-chemical. ,vol. 123, pp. 262- 268 ,(2007) , 10.1016/J.SNB.2006.08.018
Aviad Hai, Ada Dormann, Joseph Shappir, Shlomo Yitzchaik, Carmen Bartic, Gustaaf Borghs, J. P. M. Langedijk, Micha E. Spira, Spine-shaped gold protrusions improve the adherence and electrical coupling of neurons with the surface of micro-electronic devices Journal of the Royal Society Interface. ,vol. 6, pp. 1153- 1165 ,(2009) , 10.1098/RSIF.2009.0087
David Reed Markle, Misa V. Jovanovic, Solid state sensors ,(1995)