LINC01116 targets miR-520a-3p and affects IL6R to promote the proliferation and migration of osteosarcoma cells through the Jak-stat signaling pathway.
作者： Butian Zhang , Lili Yu , Ning Han , Zhenzhen Hu , Shuang Wang
DOI: 10.1016/J.BIOPHA.2018.07.119
关键词:
摘要: Abstract Objective The purpose of this study was to find out the important lncRNA-miRNA-mRNA axis and pathway in osteosarcoma (OS) through bioinformatics analysis verify biological functions lncRNA/miRNA/mRNA OS vitro vivo assays. Methods differential expression mRNAs lncRNAs were identified microarray analysis, altered pathways by GSEA. Pearson Coefficient used analyze correlations between lncRNAs. Kaplan-Meier survival preformed using patient information GEO database. Their target miRNAs predicted Targetscan miRanda database confirmed dual luciferase reporter assay. QRT-PCR utilized detected relative expressions mRNAs, IL6R protein related proteins western blot. cell viability, migration apoptosis determined MTT assay, Transwell assay flow cytometry. Tumor formation nude mice verified influence LINC01116 vivo. Results Jak-stat signaling activated tissues. positively correlated with expression. MiR-520a-3p targeted 3’-UTR IL6R. Lower levels miR-520a-3p significantly shorter patients. up-regulated while down-regulated OS. promoted viability cells, acted as a tumor suppressor. inhibitor could rescue suppressive effects si-LINC011116 si-IL6R on development. also miR-520a-3p. Down-regulation inhibited growth mice. Conclusion targeting miR-520a-3p, thus activating promoting progression
sci-hub.se 参考文章(28)
J Gong, J-P Zhang, B Li, C Zeng, K You, M-X Chen, Y Yuan, S-M Zhuang, MicroRNA-125b promotes apoptosis by regulating the expression of Mcl-1, Bcl-w and IL-6R Oncogene. ,vol. 32, pp. 3071- 3079 ,(2013) , 10.1038/ONC.2012.318
Ye Dong, Jianjun Zou, San Su, Huiyi Huang, Yanzhen Deng, Birong Wang, Weidong Li, None, MicroRNA-218 and microRNA-520a inhibit cell proliferation by downregulating E2F2 in hepatocellular carcinoma. Molecular Medicine Reports. ,vol. 12, pp. 1016- 1022 ,(2015) , 10.3892/MMR.2015.3516
M Colomiere, Alister C Ward, C Riley, Marissa K Trenerry, David Cameron-Smith, J Findlay, Leigh Ackland, Nuzhat Ahmed, None, Cross talk of signals between EGFR and IL-6R through JAK2/STAT3 mediate epithelial-mesenchymal transition in ovarian carcinomas. British Journal of Cancer. ,vol. 100, pp. 134- 144 ,(2009) , 10.1038/SJ.BJC.6604794
Weiying Zhang, Zhanping Lu, Yuen Gao, Lihong Ye, Tianqiang Song, Xiaodong Zhang, MiR-520b suppresses proliferation of hepatoma cells through targeting ten-eleven translocation 1 (TET1) mRNA Biochemical and Biophysical Research Communications. ,vol. 460, pp. 793- 798 ,(2015) , 10.1016/J.BBRC.2015.03.108
Luis Aponte-Tinao, Miguel A. Ayerza, D. Luis Muscolo, Germán L. Farfalli, Survival, recurrence, and function after epiphyseal preservation and allograft reconstruction in osteosarcoma of the knee. Clinical Orthopaedics and Related Research. ,vol. 473, pp. 1789- 1796 ,(2015) , 10.1007/S11999-014-4028-5
Matjaz Rokavec, Meryem Gülfem Öner, Huihui Li, Rene Jackstadt, Longchang Jiang, Dmitri Lodygin, Markus Kaller, David Horst, Paul K. Ziegler, Sarah Schwitalla, Julia Slotta-Huspenina, Franz G. Bader, Florian R. Greten, Heiko Hermeking, IL-6R/STAT3/miR-34a feedback loop promotes EMT-mediated colorectal cancer invasion and metastasis Journal of Clinical Investigation. ,vol. 124, pp. 1853- 1867 ,(2014) , 10.1172/JCI73531
Yan Liu, Aiguo Liu, Huameng Li, Chenglong Li, Jiayuh Lin, Celecoxib Inhibits Interleukin-6/Interleukin-6 Receptor–Induced JAK2/STAT3 Phosphorylation in Human Hepatocellular Carcinoma Cells Cancer Prevention Research. ,vol. 4, pp. 1296- 1305 ,(2011) , 10.1158/1940-6207.CAPR-10-0317
Amaia Lujambio, Scott W. Lowe, The microcosmos of cancer Nature. ,vol. 482, pp. 347- 355 ,(2012) , 10.1038/NATURE10888
Ning Wu, Yang Song, Liewen Pang, Zhiming Chen, CRCT1 regulated by microRNA-520 g inhibits proliferation and induces apoptosis in esophageal squamous cell cancer. Tumor Biology. ,vol. 37, pp. 8271- 8279 ,(2016) , 10.1007/S13277-015-4730-2
Kyung-Ran Park, Hyung-Mun Yun, Tran-Hong Quang, Hyuncheol Oh, Dong-Sung Lee, Q-Schick Auh, Eun-Cheol Kim, 4-Methoxydalbergione suppresses growth and induces apoptosis in human osteosarcoma cells in vitro and in vivo xenograft model through down-regulation of the JAK2/STAT3 pathway Oncotarget. ,vol. 7, pp. 6960- 6971 ,(2016) , 10.18632/ONCOTARGET.6873