Protection against oxidative damage in human erythrocytes and preliminary photosafety assessment of Punica granatum seed oil nanoemulsions entrapping polyphenol-rich ethyl acetate fraction

摘要: The main purpose of the present study is to evaluate ability nanoemulsion entrapping pomegranate peel polyphenol-rich ethyl acetate fraction (EAF) prepared from seed oil and medium chain triglyceride protect human erythrocyte membrane oxidative damage assess preliminary in vitro photosafety. In order phototoxic effect nanoemulsions, red blood cells (RBCs) are used as a biological model rate haemolysis photohaemolysis (5 J cm(-2) UVA) assessed vitro. level protection against caused by peroxyl radical generator AAPH RBCs well its effects on bilayer characteristics such fluidity, protein profile morphology determined. EAF-loaded nanoemulsions do not promote or photohaemolysis. Anisotropy measurements show that significantly retrain increase fluidity AAPH. SDS-PAGE analysis reveals induced degradation proteins, but reduce extension degradation. Scanning electron microscopy examinations corroborate interaction between AAPH, RBC bilayer. Our work demonstrates Punica granatum photosafe possible disturbance lipid biomembranes. Moreover it suggests these could be promising new topical products sunlight skin.