THE STRUCTURE AND BINDING CHARACTERISTICS OF SERUM AMYLOID PROTEIN (9.5S α1‐glycoprotein)
作者： R. H. Painter , I. De Escallón , A. Massey , L. Pinteric , S. B. Stern
DOI: 10.1111/J.1749-6632.1982.TB22138.X
关键词:
摘要: No difference have been observed in the properties of amyloid P-component (AP) and its serum counterpart (SAP) which might account for deposition former amyloidosis. Purified by nonselective techniques, preparations AP SAP were shown to similar molecular weights peptide composition, identical morphology electron microscope (EM) be antigenically indistinguishable. Both proteins soluble EDTA but readily precipitated presence calcium ions, forming characteristic two-dimensional arrays EM. In however, was not aggregated sedimented at 9.5S Ca2+ as did purified protein EDTA. Precipitation could prevented pretreatment with acid hydrolysates agarose or SP Sephadex, matrices has a calcium-dependent affinity. It is proposed that circulates combination low weight natural ligand prevents aggregation. While identity yet unknown, it likely resemble glycosidic subunits agarose.
sci-hub.se 参考文章(25)
Robert H. Painter, Seth N. Assimeh, David H. Bing, A simple method for the isolation of the subcomponents of the first component of complement by affinity chromatography. Journal of Immunology. ,vol. 113, pp. 225- 234 ,(1974)
R. H. Painter, L. Pinteric, D. I. C. Kells, S. N. Assimeh, The Ultrastructure of C1t, a Subcomponent of the First Component of Complement: An E.M. and Ultracentrifuge Study Journal of Immunology. ,vol. 117, pp. 79- 83 ,(1976)
R. H. Painter, Evidence That C1t (Amyloid P-Component) Is Not a Subcomponent of the First Component of Complement (C1) Journal of Immunology. ,vol. 119, pp. 2203- 2205 ,(1977)
Robert J Ziccardi, Neil R Cooper, None, The subunit composition and sedimentation properties of human C1. Journal of Immunology. ,vol. 118, pp. 2047- 2052 ,(1977)
David A. Yphantis, Equilibrium Ultracentrifugation of Dilute Solutions Biochemistry. ,vol. 3, pp. 297- 317 ,(1964) , 10.1021/BI00891A003
Arthur R. Thompson, David L. Enfield, Human plasma P component: isolation and characterization. Biochemistry. ,vol. 17, pp. 4304- 4311 ,(1978) , 10.1021/BI00613A030
Dennis E. Roark, David A. Yphantis, Studies of self-associating systems by equilibrium ultracentrifugation. Annals of the New York Academy of Sciences. ,vol. 164, pp. 245- 277 ,(1969) , 10.1111/J.1749-6632.1969.TB14043.X
Amyloid P-component (protein AP) in localized amyloidosis as revealed by an immunocytochemical method Histochemistry. ,vol. 71, pp. 171- 175 ,(1981) , 10.1007/BF00507821
M.B Pepys, A.C Dash, E.A Munn, A Feinstein, Martha Skinner, A.S Cohen, H Gewurz, A.P Osmand, R.H Painter, ISOLATION OF AMYLOID P COMPONENT (PROTEIN AP) FROM NORMAL SERUM AS A CALCIUM-DEPENDENT BINDING PROTEIN The Lancet. ,vol. 309, pp. 1029- 1031 ,(1977) , 10.1016/S0140-6736(77)91260-0
G. Mancini, A.O. Carbonara, J.F. Heremans, Immunochemical quantitation of antigens by single radial immunodiffusion Immunochemistry. ,vol. 2, pp. 235- 244 ,(1965) , 10.1016/0019-2791(65)90004-2