Divergent homeo box proteins recognize similar DNA sequences in Drosophila.

摘要: A member of a small group genes in Drosophila that define the segmentation pattern early embryo1–5 even-skipped (eve), which plays key role network interactions among genes. It appears to control morphogenesis by regulating expression gene engrailed (en)3,4, and autoregulating its own (M. Frasch M.L., preparation). Here we show these regulatory could occur at level transcription as full-length eve protein binds with high affinity specific sequences located near 5' ends en The binding sites contain least one copy 10-base pair consensus sequence: T-C-A-A-T-T-A-A-A-T. In contrast, are relatively G-C rich do not share obvious similarities sequence associated en. Other homeo box proteins can recognize both classes sites, lending support proposal involve competition different for similar cis sequences6,7.