The interaction of nonstructural protein 9 with retinoblastoma protein benefits the replication of genotype 2 porcine reproductive and respiratory syndrome virus in vitro

摘要: The nonstructural protein 9 (Nsp9) of porcine reproductive and respiratory syndrome virus (PRRSV) is a RNA-dependent RNA polymerase (RdRp) that plays vital role in viral replication. This study first demonstrated the Nsp9 genotype 2 PRRSV interacted with cellular retinoblastoma (pRb), co-localized pRb cytoplasm PRRSV-infected MARC-145 cells pulmonary alveolar macrophages (PAMs). Next, overexpression truncated was shown to inhibit replication silencing gene could facilitate cells. Finally, level confirmed be down-regulated cells, promote degradation by proteasome pathway. These findings indicate interaction Nsp benefits vitro, helping understand roles pathogenesis PRRSV.