Photochemical internalization of peptide antigens provides a novel strategy to realize therapeutic cancer vaccination

摘要: Effective priming and activation of tumor-specific CD8+ cytotoxic T lymphocytes (CTLs) is crucial for realizing the potential therapeutic cancer vaccination. This requires cytosolic antigens that feed into MHC class I presentation pathway, which not efficiently achieved with most current vaccination technologies. Photochemical internalization (PCI) provides an emerging technology to route endocytosed material cytosol cells, based on light-induced disruption endosomal membranes using a photosensitizing compound. Here, we investigated PCI as novel, minimally invasive, well-tolerated induce cancer-specific CTL responses peptide antigens. We show effectively promotes delivery antigen-presenting cells (APCs) in vitro. resulted 30-fold increase I/peptide complex formation surface presentation, subsequent 30- 100-fold more efficient antigen-specific CTLs compared alone. The effect was found be highly dependent dose treatment, where optimal doses promoted maturation immature dendritic thus also providing adjuvant effect. confirmed vivo by successful induction C57BL/6 mice following intradermal technology. new strong evidence holds great novel strategy improving outcome vaccines aimed at triggering responses.