Mechanical Stimulation of Tissue Repair in the Hydraulic Bone Chamber

摘要: A hydraulically activated bone chamber model was utilized to investigate cellular and microstructural mechanisms of mechanical adaptation during repair. Woven trabecular fibrotic granulation tissue filled the initially empty chambers by 8 weeks postimplantation into canine tibial femoral metaphyses. Without stimulation, active remodeling lamellar reconstitution marrow elements were observed between 24 weeks. In subsequent loading studies, hydraulic mechanism on one randomly chosen side 10 dogs following undisturbed The treatment applied an intermittent compressive force (18 N, 1.0 Hz, 1800 cycles/day) for durations a few days up 12 Stereological analysis three-dimensional microcomputed tomography images revealed increase in plate thickness connectivity associated with loaded repair microstructure relative unloaded contralateral controls. These alterations corresponded over 600% apparent modulus tissue. significant percentage surfaces lined osteoblasts immunopositive type I procollagen after provided further evidence stimulation matrix synthesis. local principal strains these adaptive changes estimated range from approximately -2000 +3000 mustrain using digital image-based finite element methods. This study demonstrates sensitivity cells controlled vivo stimulus identifies is introduced as efficient experimental effects biological factors regeneration.