Accurate Determination of Protein Methionine Oxidation by Stable Isotope Labeling and LC-MS Analysis
作者： Hongcheng Liu , Gomathinayagam Ponniah , Alyssa Neill , Rekha Patel , Bruce Andrien
DOI: 10.1021/AC403072W
关键词:
摘要: Methionine (Met) oxidation is a major modification of proteins, which converts Met to sulfoxide as the common product. It challenging determine level sulfoxide, because it can be generated during sample preparation and analysis an artifact. To in proteins accurately, isotope labeling LC-MS peptide mapping method was developed. residues were fully oxidized using hydrogen peroxide enriched with 18O atoms before preparation. Therefore, impossible generate artifact The molecular weight difference 2 Da between 16O atom used differentiate calculate originally. Using recombinant monoclonal antibody model protein, much lower levels detected for two susceptible this new compared typical ...
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