Targeted surface-enhanced Raman scattering nanosensors for whole-cell pH imagery.
作者： K. L. Nowak-Lovato , K. D. Rector
DOI: 10.1366/000370209787944406
关键词:
摘要: In this paper the ability to develop a gold-core, silver-shell surface-enhanced Raman scattering (SERS) nanosensor that has both targeting component as well sensing is demonstrated. The capable of through FcepsilonRI receptor-mediated endocytic pathway due adsorption 2,4-epsilon-dinitrophenol-L-lysine (DNP) ligand on nanoparticle surface. also sensitive pH changes in vesicle within range 4.5-7.5 by 4-mercaptopyridine (4-MPy) adsorbed particle and moieties do not significantly interfere with each other's function. calibrated vivo measurements rat basophil leukemia (RBL-2H3) cells standard buffer solutions using ionophore nigericin, which serves equilibrate external [H(+)] concentration cell compartments. verified beta-hexosaminidase assay. It demonstrated targeted making accurate cellular RBL-2H3 cells, out ninety minutes after addition. Whole-cell, time-lapse, hyperspectral image cubes demonstrating vesicular during receptor mediated endocytosis are
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