Effect of thrombopoietin on the development of megakaryocytes and platelets: an ultrastructural analysis.

摘要: Megakaryocytopoiesis and platelet production can be assessed with reasonable accuracy by quantitative functional analyses of circulating platelets. The evaluation megakaryocytopoiesis in culture has remained unsatisfactory, particularly because is rarely observed. In mouse systems, megakaryocytes have been identified almost entirely measurements acetyl cholinesterase, size, ploidy without concomitant assessment maturation based on such criteria as the formation granules, demarcation membranes, cytoplasmic fragmentation. availability various thrombopoietic cytokines, particular thrombopoietin (TPO), their imminent clinical use made a more detailed understanding effect differentiation MK lineage urgent. Therefore, ultrastructural were performed megakaryocyte-depleted serum-free bone marrow cultures presence TPO alone, plus other or under conditions which and/or cytokines blocked neutralizing agents. These studies show that, while that gp130 receptor subunit may function synergistically TPO, absence do not yield morphologically recognizable MK. On hand, alone able to drive full evidenced generation fragmentation into