The utility of diagnostic biopsy specimens for predictive molecular testing in colorectal cancer
作者： Wakkas Fadhil , Salih Ibrahem , Rashmi Seth , Ghada AbuAli , Krishna Ragunath
DOI: 10.1111/J.1365-2559.2012.04321.X
关键词:
摘要: Fadhil W, Ibrahem S, Seth R, AbuAli G, Ragunath K, Kaye P & Ilyas M (2012) Histopathology The utility of diagnostic biopsy specimens for predictive molecular testing in colorectal cancer Aims: If stratified medicine is to be applied the neoadjuvant setting, will have undertaken on preoperative specimens. aim this study was evaluate whether a adequately representative main tumour cancer. Methods and results: Thirty cases paired subsequent resection were randomly selected. Samples screened mutation KRAS (codons 12/13, 61, 146), BRAF (codon 600 exon 11), PIK3CA (exons 1, 9, 20), TP53 5–8), microsatellite instability, using quick multiplex consensus or standard polymerase chain reaction (PCR) protocols followed by high-resolution melting analysis. A total 570 PCR tests performed detection, identical results obtained both 569 (>99% concordance). Four (13%) showed and, all four cases, instability seen at mononucleotide markers matched specimens. Conclusions: This first show that specimens, even though they are tiny sample tumour, sufficiently use early driver mutations cancer.
sci-hub.se 参考文章(34)
S. Dighe, I. Swift, L. Magill, K. Handley, R. Gray, P. Quirke, D. Morton, M. Seymour, B. Warren, G. Brown, Accuracy of radiological staging in identifying high‐risk colon cancer patients suitable for neoadjuvant chemotherapy: a multicentre experience Colorectal Disease. ,vol. 14, pp. 438- 444 ,(2012) , 10.1111/J.1463-1318.2011.02638.X
Salih Ibrahem, Rashmi Seth, Brendan O’Sullivan, Wakkas Fadhil, Philippe Taniere, Mohammad Ilyas, Comparative analysis of pyrosequencing and QMC-PCR in conjunction with high resolution melting for KRAS/BRAF mutation detection. International Journal of Experimental Pathology. ,vol. 91, pp. 500- 505 ,(2010) , 10.1111/J.1365-2613.2010.00733.X
I. Bozic, T. Antal, H. Ohtsuki, H. Carter, D. Kim, S. Chen, R. Karchin, K. W. Kinzler, B. Vogelstein, M. A. Nowak, Accumulation of driver and passenger mutations during tumor progression Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 18545- 18550 ,(2010) , 10.1073/PNAS.1010978107
J. Yun, C. Rago, I. Cheong, R. Pagliarini, P. Angenendt, H. Rajagopalan, K. Schmidt, J. K. V. Willson, S. Markowitz, S. Zhou, L. A. Diaz, V. E. Velculescu, C. Lengauer, K. W. Kinzler, B. Vogelstein, N. Papadopoulos, Glucose deprivation contributes to the development of KRAS pathway mutations in tumor cells Science. ,vol. 325, pp. 1555- 1559 ,(2009) , 10.1126/SCIENCE.1174229
B�n�dicte Baisse, Hanifa Bouzourene, Emilia P. Saraga, Fred T. Bosman, Jean Benhattar, Intratumor genetic heterogeneity in advanced human colorectal adenocarcinoma. International Journal of Cancer. ,vol. 93, pp. 346- 352 ,(2001) , 10.1002/IJC.1343
Wakkas Fadhil, Mohammad Ilyas, Immunostaining for mismatch repair (MMR) protein expression in colorectal cancer is better and easier to interpret when performed on diagnostic biopsies. Histopathology. ,vol. 60, pp. 653- 655 ,(2012) , 10.1111/J.1365-2559.2011.04021.X
Paloma Cejas, Miriam Lopez-Gomez, Cristina Aguayo, Rosario Madero, Javier de Castro Carpeno, Cristobal Belda-Iniesta, Jorge Barriuso, Victor Moreno Garcia, Javier Larrauri, Rocío López, Enrique Casado, Manuel Gonzalez-Baron, Jaime Feliu, None, KRAS mutations in primary colorectal cancer tumors and related metastases: a potential role in prediction of lung metastasis. PLOS ONE. ,vol. 4, pp. 8199- ,(2009) , 10.1371/JOURNAL.PONE.0008199
F Di Fiore, F Blanchard, F Charbonnier, F Le Pessot, A Lamy, M P Galais, L Bastit, A Killian, R Sesboüé, J J Tuech, A M Queuniet, B Paillot, J C Sabourin, F Michot, P Michel, T Frebourg, Clinical relevance of KRAS mutation detection in metastatic colorectal cancer treated by Cetuximab plus chemotherapy. British Journal of Cancer. ,vol. 96, pp. 1166- 1169 ,(2007) , 10.1038/SJ.BJC.6603685
Antoine Italiano, Isabelle Hostein, Isabelle Soubeyran, Thibault Fabas, Daniel Benchimol, Serge Evrard, Jean Gugenheim, Yves Becouarn, René Brunet, Marianne Fonck, Eric François, Marie-Christine Saint-Paul, Florence Pedeutour, KRAS and BRAF Mutational Status in Primary Colorectal Tumors and Related Metastatic Sites: Biological and Clinical Implications Annals of Surgical Oncology. ,vol. 17, pp. 1429- 1434 ,(2010) , 10.1245/S10434-009-0864-Z
N Knijn, L J M Mekenkamp, M Klomp, M E Vink-Börger, J Tol, S Teerenstra, J W R Meijer, M Tebar, S Riemersma, J H J M van Krieken, C J A Punt, I D Nagtegaal, KRAS mutation analysis: a comparison between primary tumours and matched liver metastases in 305 colorectal cancer patients British Journal of Cancer. ,vol. 104, pp. 1020- 1026 ,(2011) , 10.1038/BJC.2011.26