Impaired CENP-E Function Renders Large Chromosomes More Vulnerable to Congression Failure.

摘要: It has recently emerged that human chromosomes vary between one another in terms of features impact their behaviour during impaired chromosome segregation, leading to non-random aneuploidy the daughter cell population. During process congression metaphase plate, movement is guided by kinesin-like proteins, among which centromere-associated protein E (CENP-E) important transport along microtubules mitotic spindle. known inhibition CENP-E notably impairs alignment for a subset chromosomes, particularly those positioned close centrosome at nuclear envelope breakdown ('polar chromosomes'); it is, however, not clear whether identity could influence this process. Since popular strategy model induce defects (for example combining inhibitors with checkpoint abrogation), variance efficiency might landscape and subsequent fates. By immunofluorescence, live imaging fluorescence situ hybridisation, we investigated polar dependency upon CENP-E-mediated cells. We observed bias related size, larger more sensitive inhibition. This likely due two contributing factors; an initial propensity be peripheral thus rely function migrate additionally specific chromosomes' ability congress from state. These findings may help explain persistence following disruption, also have implications spectrum generated treatments manipulate function.