Immunological and biochemical properties of transverse tubule membranes isolated from rabbit skeletal muscle.
作者： M. Rosemblatt , C. Hidalgo , C. Vergara , N. Ikemoto
DOI: 10.1016/S0021-9258(18)43399-6
关键词:
摘要: A new method for isolating transverse tubule membranes from rabbit skeletal muscle has been developed. This procedure the advantage of being mild, fast, and producing with good yields a purified membrane fraction. The are by discontinuous sucrose density centrifugation after loading contaminating light sarcoplasmic reticulum vesicles calcium phosphate in presence ATP. Immunofluorescence staining cryostat sections psoas goat antibodies directed against shows that reacting antigens distributed at boundary I bands myofibrils where tubules localized mammalian muscle. showed no cross-reactivity reticulum, nor did they show any fluorescence plasma membrane, indicating isolated indeed originate tubules. fraction characteristic protein composition distinguishable much higher cholesterol content than crude microsomes, phospholipid about twice as high membrane. distinct contents sphingomyelin phosphatidylserine. Mg2+-activated ATPase undergoes 20-30-fold increase specific activity during purification. levels Ca2+-ATPase present remain comparable to those even extensive removal latter.
unab.cl
sci-hub.st 参考文章(28)
Sidney Fleischer, Becca Fleischer, [70] Removal and binding of polar lipids in mitochondria and other membrane systems Methods in Enzymology. ,vol. 10, pp. 406- 433 ,(1967) , 10.1016/0076-6879(67)10073-6
Y H Lau, A H Caswell, J P Brunschwig, Isolation of transverse tubules by fractionation of triad junctions of skeletal muscle. Journal of Biological Chemistry. ,vol. 252, pp. 5565- 5574 ,(1977) , 10.1016/S0021-9258(19)63388-0
Y.H. Lau, A.H. Caswell, J.P. Brunschwig, R.j. Baerwald, M. Garcia, Lipid analysis and freeze-fracture studies on isolated transverse tubules and sarcoplasmic reticulum subfractions of skeletal muscle. Journal of Biological Chemistry. ,vol. 254, pp. 540- 546 ,(1979) , 10.1016/S0021-9258(17)37949-8
M. Michalak, K.P. Campbell, D.H. MacLennan, Localization of the high affinity calcium binding protein and an intrinsic glycoprotein in sarcoplasmic reticulum membranes. Journal of Biological Chemistry. ,vol. 255, pp. 1317- 1326 ,(1980) , 10.1016/S0021-9258(19)86032-5
C. Hidalgo, N. Ikemoto, Disposition of proteins and aminophospholipids in the sarcoplasmic reticulum membrane. Journal of Biological Chemistry. ,vol. 252, pp. 8446- 8454 ,(1977) , 10.1016/S0021-9258(19)75239-9
Charles C Allain, Lucy S Poon, Cicely S G Chan, W Richmond, Paul C Fu, Enzymatic determination of total serum cholesterol. Clinical Chemistry. ,vol. 20, pp. 470- 475 ,(1974) , 10.1093/CLINCHEM/20.4.470
M. Endo, Calcium release from the sarcoplasmic reticulum Physiological Reviews. ,vol. 57, pp. 71- 108 ,(1977) , 10.1152/PHYSREV.1977.57.1.71
A O Jorgensen, V Kalnins, D H MacLennan, Localization of sarcoplasmic reticulum proteins in rat skeletal muscle by immunofluorescence. Journal of Cell Biology. ,vol. 80, pp. 372- 384 ,(1979) , 10.1083/JCB.80.2.372
R.L. Barchi, J.B. Weigele, D.M. Chalikian, L.E. Murphy, Muscle surface membranes. Preparative methods affect apparent chemical properties and neurotoxin binding Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 550, pp. 59- 76 ,(1979) , 10.1016/0005-2736(79)90115-9
Keith R. Porter, George E. Palade, STUDIES ON THE ENDOPLASMIC RETICULUM : III. ITS FORM AND DISTRIBUTION IN STRIATED MUSCLE CELLS Journal of Cell Biology. ,vol. 3, pp. 269- 300 ,(1957) , 10.1083/JCB.3.2.269