Triglycidylamine Crosslinking of Porcine Aortic Valve Cusps or Bovine Pericardium Results in Improved Biocompatibility, Biomechanics, and Calcification Resistance: Chemical and Biological Mechanisms

摘要: We investigated a novel polyepoxide crosslinker that was hypothesized to confer both material stabilization and calcification resistance when used prepare bioprosthetic heart valves. Triglycidylamine (TGA) synthesized via reacting epichlorhydrin NH3. TGA crosslink porcine aortic cusps, bovine pericardium, type I collagen. Control materials were crosslinked with glutaraldehyde (Glut). TGA-pretreated had shrink temperatures comparable Glut fixation. However, crosslinking conferred significantly greater collagenase than pretreatment, improved biomechanical compliance. Sheep valve interstitial cells grown on collagen did not calcify, whereas sheep control substrates calcified extensively. Rat subdermal implants (porcine cusps/bovine pericardium) pretreated demonstrated less implants. Investigations of extracellular matrix proteins associated calcification, metalloproteinases (MMPs) 2 9, tenascin-C, osteopontin, revealed MMP-9 tenascin-C reduced expression in vitro vivo compared controls, osteopontin MMP-2 affected. pretreatment heterograft biomaterials results stability Glut, confers properties superior crosslinking, demonstrates significant resistance.