Detection of gp43 of Paracoccidioides brasiliensis by the loop‐mediated isothermal amplification (LAMP) method
作者： Makoto Miyaji , Katsuhiko Kamei , Giannina Ricci , Marcello Franco , Kazuko Nishimura
DOI: 10.1016/J.FEMSLE.2004.03.015
关键词:
摘要: Paracoccidioidomycosis is a deep mycosis caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and prevalent in Latin American countries. We detected species specific gp43 gene of P. loop-mediated isothermal amplification (LAMP) 22 clinical seven armadillo-derived isolates. The amplified DNA appeared as ladder with banding pattern. advantage LAMP method speed; only 3 h were necessary for identification organism diagnosis disease. also able to obtain positive results from extracted paraffin-embedded tissue sample paracoccidioidomycosis, suggesting that this may achieve application near future.
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Ralf Bialek, Aida Ibricevic, Christian Aepinus, Laura K. Najvar, Annette W. Fothergill, Jürgen Knobloch, John R. Graybill, Detection of Paracoccidioides brasiliensis in Tissue Samples by a Nested PCR Assay Journal of Clinical Microbiology. ,vol. 38, pp. 2940- 2942 ,(2000) , 10.1128/JCM.38.8.2940-2942.2000
Yasuyoshi Mori, Kentaro Nagamine, Norihiro Tomita, Tsugunori Notomi, Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochemical and Biophysical Research Communications. ,vol. 289, pp. 150- 154 ,(2001) , 10.1006/BBRC.2001.5921
K. Nagamine, T. Hase, T. Notomi, Accelerated reaction by loop-mediated isothermal amplification using loop primers. Molecular and Cellular Probes. ,vol. 16, pp. 223- 229 ,(2002) , 10.1006/MCPR.2002.0415
T. Iwamoto, T. Sonobe, K. Hayashi, Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples. Journal of Clinical Microbiology. ,vol. 41, pp. 2616- 2622 ,(2003) , 10.1128/JCM.41.6.2616-2622.2003
Angela Restrepo M, The ecology of Paracoccidioides brasiliensis: a puzzle still unsolved. Medical Mycology. ,vol. 23, pp. 323- 334 ,(1985) , 10.1080/00362178585380481
Patrícia S. Cisalpino, Rosana Puccia, Lucy M. Yamauchi, Maria I. N. Cano, J. Franco da Silveira, Luiz R. Travassos, CLONING, CHARACTERIZATION, AND EPITOPE EXPRESSION OF THE MAJOR DIAGNOSTIC ANTIGEN OF PARACOCCIDIOIDES BRASILIENSIS Journal of Biological Chemistry. ,vol. 271, pp. 4553- 4560 ,(1996) , 10.1074/JBC.271.8.4553
T. DE BRITO, G. S. SANDHU, B. C. KLINE, R. A. ALEFF, M. P. SANDOVAL, R. T. SANTOS, A. A. H. BRANDÃO, C. S. LACAZ, In situ hybridization in paracoccidioidomycosis Medical Mycology. ,vol. 37, pp. 207- 211 ,(1999) , 10.1046/J.1365-280X.1999.00222.X
S. DÍEZ, E.A. GARCIA, P.A. PINO, S. BOTERO, G.G. CORREDOR, L.A. PERALTA, J.H. CASTAÑO, A. RESTREPO, J.G. McEWEN, PCR with Paracoccidioides brasiliensis specific primers: potential use in ecological studies Revista Do Instituto De Medicina Tropical De Sao Paulo. ,vol. 41, pp. 351- 358 ,(1999) , 10.1590/S0036-46651999000600004
Junko Fujio, Kazuko Nishimura, Makoto Miyaji, Epidemiological Survey of the Imported Mycoses in Japan. Nippon Ishinkin Gakkai Zasshi. ,vol. 40, pp. 103- 109 ,(1999) , 10.3314/JJMM.40.103
Ayako Sano, Koji Yokoyama, Miki Tamura, Yuzuru Mikami, Ichiro Takahashi, Kazutaka Fukushima, Makoto Miyaji, Kazuko Nishimura, Detection of gp43 and ITS1-5.8S-ITS2 Ribosomal RNA Genes of Paracoccidioides brasiliensis in Paraffin-embedded Tissue. Nippon Ishinkin Gakkai Zasshi. ,vol. 42, pp. 23- 27 ,(2001) , 10.3314/JJMM.42.23