Secretion and biological activities of heparin-binding growth-associated molecule. Neurite outgrowth-promoting and mitogenic actions of the recombinant and tissue-derived protein.
作者： E Raulo , I Julkunen , J Merenmies , R Pihlaskari , H Rauvala
DOI: 10.1016/S0021-9258(19)49925-0
关键词:
摘要: The cDNA for the developmentally regulated, neurite outgrowth-promoting protein HB-GAM (heparin-binding growth-associated molecule) was recently cloned and shown to encode a novel lysine-rich sequence that is homologous with retinoic acid-induced sequences suggested function in cell differentiation (Merenmies, J., Rauvala, H. (1990) J. Biol. Chem. 265, 16721-16724). same found mitogenic pleiotrophin (Li, Y.-S., Milner, P. G., Chauhan, A. K., Watson, M. A., Hoffman, R. M., Kodner, C. Milbrandt, Deuel, T. F. Science 250, 1690-1694). In this study, we have constructed recombinant baculovirus using encodes putative preprotein of HB-GAM. secretion signal cleaved off expression system, rapidly secreted culture medium. Recombinant purified from medium retains biochemical characteristics activity tissue-derived protein. Studies on suggest functions as an extracellular matrix-associated enhances axonal growth perinatal cerebral neurons rat. Since predicted amino acid has been ascribed protein, activities fractions were also studied. did not display any significant activity, suggesting preparations may contain other heparin-binding factors. We identified brain-derived 17-kDa (p17) detached heparin by slightly higher salt concentration compared p17 structurally distinct responsible actions fractions.
sci-hub.st 参考文章(42)
Peyrat Jp, Leroy-Martin B, Dusanter-Fourt I, Bonneterre J, Demaille A, Djiane J, Characterization of insulin-like growth factor 1 receptors (IGF1-R) in human breast cancer cell lines. Bulletin Du Cancer. ,vol. 76, pp. 311- 319 ,(1989)
S K Akiyama, K M Yamada, The interaction of plasma fibronectin with fibroblastic cells in suspension. Journal of Biological Chemistry. ,vol. 260, pp. 4492- 4500 ,(1985) , 10.1016/S0021-9258(18)89291-2
N.H. Guo, H.C. Krutzsch, T Vogel, D.D. Roberts, Interactions of a laminin-binding peptide from a 33-kDa protein related to the 67-kDa laminin receptor with laminin and melanoma cells are heparin-dependent. Journal of Biological Chemistry. ,vol. 267, pp. 17743- 17747 ,(1992) , 10.1016/S0021-9258(19)37106-6
A M Krensky, A K Bhan, T A Springer, T J Flotte, S J Burakoff, M E Hemler, J L Strominger, F Sanchez-Madrid, Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. Journal of Immunology. ,vol. 132, pp. 3011- 3018 ,(1984)
Nenghua Guo, Vishva M. Dixit, Yoji Shimizu, Rachel Yabkowitz, David D. Roberts, Activated T-cell adhesion to thrombospondin is mediated by the alpha 4 beta 1 (VLA-4) and alpha 5 beta 1 (VLA-5) integrins. Journal of Immunology. ,vol. 151, pp. 149- 158 ,(1993)
N.H. Guo, H.C. Krutzsch, E Nègre, V.S. Zabrenetzky, D.D. Roberts, Heparin-binding peptides from the type I repeats of thrombospondin. Structural requirements for heparin binding and promotion of melanoma cell adhesion and chemotaxis. Journal of Biological Chemistry. ,vol. 267, pp. 19349- 19355 ,(1992) , 10.1016/S0021-9258(18)41781-4
Tikva Vogel, Neng-Hua Guo, Henry C. Krutzsch, Diane A. Blake, Jacob Hartman, Simona Mendelovitz, Amos Panet, David D. Roberts, Modulation of endothelial cell proliferation, adhesion, and motility by recombinant heparin‐binding domain and synthetic peptides from the type I repeats of thrombospondin Journal of Cellular Biochemistry. ,vol. 53, pp. 74- 84 ,(1993) , 10.1002/JCB.240530109
N. H. Guo, H. C. Krutzsch, E. Negre, T. Vogel, D. A. Blake, D. D. Roberts, Heparin- and sulfatide-binding peptides from the type I repeats of human thrombospondin promote melanoma cell adhesion. Proceedings of the National Academy of Sciences. ,vol. 89, pp. 3040- 3044 ,(1992) , 10.1073/PNAS.89.7.3040
J Lawler, R Weinstein, R O Hynes, Cell attachment to thrombospondin: the role of ARG-GLY-ASP, calcium, and integrin receptors. Journal of Cell Biology. ,vol. 107, pp. 2351- 2361 ,(1988) , 10.1083/JCB.107.6.2351
G P Tuszynski, M A Kowalska, Thrombospondin-induced adhesion of human platelets. Journal of Clinical Investigation. ,vol. 87, pp. 1387- 1394 ,(1991) , 10.1172/JCI115144