AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging.

摘要: It is known that the presence of fluorophores can influence dynamics molecular processes. Despite this, an affordable technique to control fluorophore distribution within sample, as well rise unpredictable anomalous processes induced by itself, missing. We coupled a stimulated emission depletion (STED) microscope with atomic force investigate formation amyloid aggregates. In particular, we studied in vitro aggregation insulin and two alloforms β peptides. followed standard methods induce label molecules at different dye-to-protein ratios. Only fraction fibrillar aggregates was displayed STED images, indicating labeled did not participate indistinctly process. This finding demonstrates follow only selected pathways aggregation, among multiple are present reaction.