Non-invasive monitoring of red blood cells during cold storage using handheld Raman spectroscopy.

摘要: Background The current best practices allow for the red blood cells (RBCs) to be stored prolonged periods in banks worldwide. However, due individual-related variability donated and RBCs continual degradation within transfusion bags, quality of varies considerably. There is currently no method assessing product without compromising sterility unit. This study demonstrates feasibility monitoring storage lesion situ while maintaining using an optical approach. Study design methods A handheld spatially offset Raman spectroscopy (RS) device was employed non-invasively monitor hemolysis metabolic changes 12 cell concentrate (RCC) units standard sealed bags over 7 weeks cold storage. analyzed parallel by biochemical (chemical analysis, spectrophotometry, hematology analysis) RS measurements, which were then correlated through multisource correlation analysis. Results bands lactate (857 cm-1 ), glucose (787 (1003 ) found correlate strongly with bioanalytical data length storage, values 0.98 (95% confidence interval [CI]: 0.86-1.00; p = .0001), 0.95 CI: 0.71-0.99; .0008) 0.97 0.79-1.00; .0004) respectively. Discussion potential collecting information on clinical breaching technology. could significantly benefit control RCC units, patient safety inventory management hospitals.