DNA Assembly in 3D Printed Fluidics

摘要: The process of connecting genetic parts—DNA assembly—is a foundational technology for synthetic biology. Microfluidics present an attractive solution minimizing use costly reagents, enabling multiplexed reactions, and automating protocols by integrating multiple protocol steps. However, microfluidics fabrication operation can be expensive requires expertise, limiting access to the technology. With advances in commodity digital tools, it is now possible directly print fluidic devices supporting hardware. 3D printed micro- millifluidic are inexpensive, easy make quick produce. We demonstrate Golden Gate DNA assembly 3D-printed fluidics with reaction volumes as small 490 nL, channel widths fine 220 microns, per unit part costs ranging from $0.61 $5.71. A syringe pump accompanying programmable software interface was designed fabricated operate devices. Quick turnaround inexpensive materials allowed rapid exploration device parameters, demonstrating manufacturing paradigm designing fabricating hardware